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Mixture was then e xtr acted with pr e-cooled eth yl aceta te (100 mL × 3). Acetone dicarboxylic acid a water bath, the temperature of which was adjusted to the 30°C. H Each step is through crystallization or simple extraction and without column chromatography. Additionally, ... Read Full Source

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E1447 Vol 5, Iss 8, Apr 20, 2015 - BIO-PROTOCOL.ORG
13C Kinetic Labeling and Extraction of Metabolites from Adherent Mammalian Cells 3. Water (LC-MS Ultra CHROMASOLV) (Sigma-Aldrich, catalog number: 14263) http://www.bio-protocol.org/e1447 Vol 5, Iss 8, ... Document Viewer